Regulatory
Part:BBa_K259007:Design
Designed by: Petros Mina Group: iGEM09_BCCS-Bristol (2009-09-09)
AraC Promoter fused with RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 294
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 125
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 107
Design Notes
The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.
Source
The AraC promoter can be found naturally at 1.52min of the E.coli genome ([http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene number EG10054].
The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page here.